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phospho alk  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phospho alk
    Phospho Alk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho alk/product/Cell Signaling Technology Inc
    Average 94 stars, based on 31 article reviews
    phospho alk - by Bioz Stars, 2026-03
    94/100 stars

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    Optimized protocols for ALK IHC assays using the BP6165 concentrated antibody on the LYNX480 PLUS platform.

    Journal: Pathology and Oncology Research

    Article Title: A novel automated IHC staining system for quality control application in ALK immunohistochemistry testing

    doi: 10.3389/pore.2025.1611964

    Figure Lengend Snippet: Optimized protocols for ALK IHC assays using the BP6165 concentrated antibody on the LYNX480 PLUS platform.

    Article Snippet: The rabbit monoclonal anti-ALK antibody BP6165 (catalog number: I1153, Biolynx) assay was performed using the LYNX480 PLUS System with conventional 3,3′-diaminobenzidine (DAB) staining (no amplification).

    Techniques:

    ALK staining patterns on lung adenocarcinoma samples using the BP6165 concentrated antibody on the LYNX480 PLUS platform (A, C) compared with the ALK (D5F3) CDx on the VENTANA BenchMark XT platform (gold standard) (B, D) .

    Journal: Pathology and Oncology Research

    Article Title: A novel automated IHC staining system for quality control application in ALK immunohistochemistry testing

    doi: 10.3389/pore.2025.1611964

    Figure Lengend Snippet: ALK staining patterns on lung adenocarcinoma samples using the BP6165 concentrated antibody on the LYNX480 PLUS platform (A, C) compared with the ALK (D5F3) CDx on the VENTANA BenchMark XT platform (gold standard) (B, D) .

    Article Snippet: The rabbit monoclonal anti-ALK antibody BP6165 (catalog number: I1153, Biolynx) assay was performed using the LYNX480 PLUS System with conventional 3,3′-diaminobenzidine (DAB) staining (no amplification).

    Techniques: Staining

    Positive percentage agreement (PPA) and negative percentage agreement (NPA) for ALK IHC assays using the BP6165 concentrated antibody on the LYNX480 PLUS platform compared with the ALK (D5F3) CDx Kit on the VENTANA BenchMark XT platform (gold standard).

    Journal: Pathology and Oncology Research

    Article Title: A novel automated IHC staining system for quality control application in ALK immunohistochemistry testing

    doi: 10.3389/pore.2025.1611964

    Figure Lengend Snippet: Positive percentage agreement (PPA) and negative percentage agreement (NPA) for ALK IHC assays using the BP6165 concentrated antibody on the LYNX480 PLUS platform compared with the ALK (D5F3) CDx Kit on the VENTANA BenchMark XT platform (gold standard).

    Article Snippet: The rabbit monoclonal anti-ALK antibody BP6165 (catalog number: I1153, Biolynx) assay was performed using the LYNX480 PLUS System with conventional 3,3′-diaminobenzidine (DAB) staining (no amplification).

    Techniques:

    IHC staining results of lung adenocarcinoma samples, ALK positive and negative cell controls by applying different antibody concentrations. (A1–A4) Lung adenocarcinoma samples stained with BP6165 at different concentrations (original magnification: ×20). (A1) The highest concentration with an antibody dilution rate of 1:25 shows nonspecific staining on lymphocytes; (A2) antibody dilution rate of 1:200 shows an optimal staining pattern; (A3) antibody dilution rate of 1:1,600 shows weaker staining than the optimal staining; (A4) dilution rate of 1:6400 shows even weaker staining. (B1–B4) ALK positive controls in liquid form stained with BP6165 at different concentrations (original magnification: ×20). (B1, B2) Both antibody dilution rates of 1:25 and 1:200 show a standard staining pattern; (B3) antibody dilution rate of 1:1,600 shows a lower positive rate, away from the standard staining pattern; (B4) antibody dilution rate of 1:6400 shows even weaker staining. (C1–C4) ALK negative controls in liquid form stained with BP6165 at different concentrations (original magnification: ×20). (C1) antibody dilution rate of 1:25 shows “background staining;” (C2–C4) dilution rates of 1:200, 1:1600, and 1:6400 show standard negative results.

    Journal: Pathology and Oncology Research

    Article Title: A novel automated IHC staining system for quality control application in ALK immunohistochemistry testing

    doi: 10.3389/pore.2025.1611964

    Figure Lengend Snippet: IHC staining results of lung adenocarcinoma samples, ALK positive and negative cell controls by applying different antibody concentrations. (A1–A4) Lung adenocarcinoma samples stained with BP6165 at different concentrations (original magnification: ×20). (A1) The highest concentration with an antibody dilution rate of 1:25 shows nonspecific staining on lymphocytes; (A2) antibody dilution rate of 1:200 shows an optimal staining pattern; (A3) antibody dilution rate of 1:1,600 shows weaker staining than the optimal staining; (A4) dilution rate of 1:6400 shows even weaker staining. (B1–B4) ALK positive controls in liquid form stained with BP6165 at different concentrations (original magnification: ×20). (B1, B2) Both antibody dilution rates of 1:25 and 1:200 show a standard staining pattern; (B3) antibody dilution rate of 1:1,600 shows a lower positive rate, away from the standard staining pattern; (B4) antibody dilution rate of 1:6400 shows even weaker staining. (C1–C4) ALK negative controls in liquid form stained with BP6165 at different concentrations (original magnification: ×20). (C1) antibody dilution rate of 1:25 shows “background staining;” (C2–C4) dilution rates of 1:200, 1:1600, and 1:6400 show standard negative results.

    Article Snippet: The rabbit monoclonal anti-ALK antibody BP6165 (catalog number: I1153, Biolynx) assay was performed using the LYNX480 PLUS System with conventional 3,3′-diaminobenzidine (DAB) staining (no amplification).

    Techniques: Immunohistochemistry, Staining, Concentration Assay